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347302 antihuman cyclin b1 gns 1 164dy fluidigm cat  (fluidigm)


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    fluidigm 347302 antihuman cyclin b1 gns 1 164dy fluidigm cat
    347302 Antihuman Cyclin B1 Gns 1 164dy Fluidigm Cat, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/347302 antihuman cyclin b1 gns 1 164dy fluidigm cat/product/fluidigm
    Average 94 stars, based on 20 article reviews
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    Image Search Results


    Expression levels of various markers in differentiated hPSC-derived myogenic progenitors treated with rat sera. The expression of Pax7 and Myogenin (MyoG) proteins were analyzed in the differentiated cells for 3 days with 20% young or aged rat sera. (A) The type of supplemented sera did not alter the percentage of total myogenic cells (expressing PAX7, MyoG, or both) (Student’s t -test, t = 1.115, df = 10, p = 0.291). There was no difference in the number (B) and proportions (C) of quiescent, activating and activated myogenic progenitors out of total cells (represented by cells expressing PAX7 + /MyoG − , PAX7 + /MyoG + and PAX7 − /MyoG + , respectively) (2-way ANOVA, F 1,30 = 1.958, p = 0.172 for (B) ; Student’s t -test, t < 0.001 df = 2, p > 0.999 for (C) . (D) On Day 7 of terminal differentiation, cells supplemented with aged rat sera showed an increased level of a cell proliferation marker Ki67 (t = 9.357, df = 10, p < 0.001; by unpaired two-tailed Student’s t-test). (E) Western blot analysis showed increased cyclin B1 and CDK 2 expression in hPSC-derived myogenic progenitors differentiated in 20% aged rat sera for 2 weeks. Beta-actin served as loading control. n = 6 for each group * p < 0.05.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Cellular and transcriptomic changes by the supplementation of aged rat serum in human pluripotent stem cell-derived myogenic progenitors

    doi: 10.3389/fcell.2024.1481491

    Figure Lengend Snippet: Expression levels of various markers in differentiated hPSC-derived myogenic progenitors treated with rat sera. The expression of Pax7 and Myogenin (MyoG) proteins were analyzed in the differentiated cells for 3 days with 20% young or aged rat sera. (A) The type of supplemented sera did not alter the percentage of total myogenic cells (expressing PAX7, MyoG, or both) (Student’s t -test, t = 1.115, df = 10, p = 0.291). There was no difference in the number (B) and proportions (C) of quiescent, activating and activated myogenic progenitors out of total cells (represented by cells expressing PAX7 + /MyoG − , PAX7 + /MyoG + and PAX7 − /MyoG + , respectively) (2-way ANOVA, F 1,30 = 1.958, p = 0.172 for (B) ; Student’s t -test, t < 0.001 df = 2, p > 0.999 for (C) . (D) On Day 7 of terminal differentiation, cells supplemented with aged rat sera showed an increased level of a cell proliferation marker Ki67 (t = 9.357, df = 10, p < 0.001; by unpaired two-tailed Student’s t-test). (E) Western blot analysis showed increased cyclin B1 and CDK 2 expression in hPSC-derived myogenic progenitors differentiated in 20% aged rat sera for 2 weeks. Beta-actin served as loading control. n = 6 for each group * p < 0.05.

    Article Snippet: The membrane was immunoblotted with anti-human cyclin B1 antibody (1:1000; 4,138, Cell Signaling Technology, Danvers, MA) and Cell Cycle WB Cocktail (1:250; ab136810, Abcam, Cambridge, MA) containing primary antibodies targeting phospho-cdk2 Tyr15 and beta-actin, followed by secondary antibody conjugated with horseradish peroxidase (anti-rabbit IgG HRP; Promega, Madison, WI).

    Techniques: Expressing, Derivative Assay, Marker, Two Tailed Test, Western Blot, Control